Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Stromal softness confines pancreatic cancer growth through lysosomal-cathepsin mediated YAP1 degradation

doi: 10.1007/s00018-024-05466-y

Figure Lengend Snippet: PDAC tumorigenesis and metastasis are promoted in the fibrotic liver ( A ) Schematic representation of the experimental design used to determine the impact of liver fibrosis/stiffness on tumorigenicity. The mice were treated with or without TAA for 4 weeks, followed by inoculation of UN-KPC961 cells for 2 weeks ( n = 6). ( B ) Gross examination (upper row) and H&E staining (lower row) of control and tumor-bearing livers. Scale bar = 500 μm. ( C ) Quantitation of tumor numbers in normal or fibrotic liver sections. ( D ) H&E staining of normal or fibrotic liver sections, scale bar = 200 μm. ( E ) Immunohistochemical staining of type-I collagen, Ki67 and CK19 in normal or fibrotic liver sections, scale bar = 100 μm. ( F ) Immunohistochemical staining of YAP1 and CtsL in normal or fibrotic liver sections, scale bar = 100 μm. ( G ) Immunofluorescence staining of CtsL and YAP1 in PanIN and adjacent hepatic parenchyma. The PanIN ducts are indicated by brown dashed lines, and malignant cells are indicated by pink arrows, scale bar = 25 μm. The values are presented as mean ± SD; ** p < 0.01

Article Snippet: Images of CTSL-stained PDAC tissue and survival analysis of PDAC patients were obtained from the Human Protein Atlas.

Techniques: Staining, Control, Quantitation Assay, Immunohistochemical staining, Immunofluorescence

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Stromal softness confines pancreatic cancer growth through lysosomal-cathepsin mediated YAP1 degradation

doi: 10.1007/s00018-024-05466-y

Figure Lengend Snippet: High expression of lysosomal-cathepsins correlates with YAP1 down-regulation, less fibrosis and survival of PDAC patients ( A ) Representative immunohistochemistry images of YAP1 and CTSL staining in pancreatic tissues from PDAC patients ( n = 30 for PDAC, n = 29 for control). ( B ) Semi quantitation of CTSL staining in PDAC cells vs. the control cells was determined by densitometry analysis. ( C ) Fibrotic stiffness in human PDAC tissues was determined as expression of tissue transglutaminase 2 (TGM2) in adjacent to the tumor ducts by immunohistochemistry. ( D ) A typical image of human pancreatic cancer slide stained for CTSL, from the Human Protein Atlas database . Fibrotic stiffness is indicated by dashed green circles. ( E ) Decreased expression of CTSL is associated with poor prognosis in PDAC patients (from the Human Protein Atlas database ). The values are presented as mean ± SD; ** p < 0.01. Scale bar = 50 μm

Article Snippet: Images of CTSL-stained PDAC tissue and survival analysis of PDAC patients were obtained from the Human Protein Atlas.

Techniques: Expressing, Immunohistochemistry, Staining, Control, Quantitation Assay

Journal: Scientific Reports

Article Title: Activation of STING in pancreatic cancer-associated fibroblasts exerts an antitumor effect by enhancing tumor immunity

doi: 10.1038/s41598-024-68061-y

Figure Lengend Snippet: STING expression in human PDAC stroma and CAFs. ( a ) Groups categorized by positive, weakly positive, and negative STING expression in PDAC stroma. Representative IHC analysis of STING using a human PDAC tissue array (scale bar, 100 μm). ( b ) Percentages of the indicated groups in 40 PDAC samples. ( c ) Co-immunofluorescence staining for α-SMA (red) and STING (green) in STING positive or weakly positive or negative in stroma. ( d ) 5-AZA (0.1 nM) was administered to CAF1 and CAF2 derived from murine PDACs and incubated for 24 h.

Article Snippet: The human PDAC tissue array was purchased commercially from TissueArray.com (No.PA482a).

Techniques: Expressing, Immunofluorescence, Staining, Derivative Assay, Incubation